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Florence Barker was born on November 22, 1891, in Los Angeles, California. Her father, Norman Barker, worked as a farmer, and she had four siblings. As a teenager, she began acting with stock companies. She fell in love with actor Joe De Grasse, who was 18 years older than her; they lived together, and he helped guide her career. In 1908, she made her film debut in D.W. Griffith's "An Awful Moment." Over the following three years, she appeared in more than 50 films, including "The Course of True Love," "The Diamond Star," and "The Newlyweds" alongside Mary Pickford. She became one of the first American actresses to work in European films. Regarding acting, she remarked, "I love the work and would advise any actress to make a try for the pictures. The work is arduous to be sure. No one ever attained success unless it was accomplished by close and concerted application." In 1912, the talented young actress was signed by Powers Players and was cast in leading roles in comedies such as "Her Yesterday" and "The Petticoat Detective." Her performances received positive reviews, pointing to a promising future. She also wrote the script for her 1912 comedy "Priscilla's Comedy." While visiting her mother in early 1913, she fell ill. Sadly, on February 15, 1913, she died of pneumonia at just 21 years old. She was cremated, and her ashes were interred at Evergreen Cemetery in Los Angeles, California.